Violet Red Bile Agar. M Violet Red Bile Agar is selective medium used for the isolation, detection and enumeration of coli-aerogenes bacteria in water, milk. Violet Red Bile Agar (VRB Agar). APHA / COMPF / IDF-FIL / ISO Medium for the detection and enumeration of coliforms in milk and other dairy products. Violet Red Bile Agar is used for the enumeration of coliforms in food and the presumptive test for coliforms uses Violet Red Bile Agar, (VRBA).

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There should be enough water in the waterbath to reach the media line.

U Violet Red Bile Agar, 8oz. Melt agar by placing in a boiling agra until liquified. This product is not intended to be used for the diagnosis of human disease. Lactose-fermenting microorganisms, including coliforms, produce pink to red colonies that are generally surrounded by a reddish zone of precipitated bile.

Violet Red Bile Agar (VRBA) – Lab M

Standard microbiological supplies and equipment such as loops, slides, staining supplies, Lauryl Sulfate Broth, other culture media, microscopes, incinerators, and incubators, etc. Aseptically inoculate agar surface with 0. Growth; pink to vrh colonies, may have a slight precipitate around colonies colonies.


For vrbb bottled media: Place a 1ml inoculation into a sterile petri plate. Lactose-fermenting microorganisms produce pink to red colonies that are generally surrounded by a reddish zone of precipitated bile.


Heat in waterbath until melted. Spread the dilution evenly over the surface of the medium. Prepare decimal dilutions in sterile diluent to obtain CFU per plate.

A covered waterbath will help to reach and maintain the temperature. Media should not be used if there are any signs of deterioration shrinking, cracking, or discolorationcontamination, or if the expiration date has passed.

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Using a sterile spreader device, distribute the inoculum evenly over the agar surface. Hardy Diagnostics Violet Red Bile Agar is recommended for the detection of coliforms in food or dairy products. Consult listed references for information on sample collection. Violet Red Bile Agar contains a low concentration of bile salts, and therefore is not completely specific for enterics.

Surface colonies of Escherichia coli appear as entire-edged colonies, while deep colonies appear lens-shaped.

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Carefully swirl the plate to mix the inoculum evenly. Consult listed references for information concerning inoculation procedures. Non-lactose-fermenting microorganisms result in colorless colonies.


Neutral red qgar employed as the pH indicator. Enterococci may grow, and if so, usually appear pinpoint in size and rose colored. Colonies of Enterobacter aerogenes often appear mucoid and pinkish in color.

Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing. Allow medium to warm to room temperature prior to inoculation. Alternatively, a covered, boiling waterbath degrees C.

The medium contains bile salts and crystal violet which serve as inhibitory agents toward some gram-positive microorganisms, especially staphylococci. The medium is recommended for use in the microbiological analysis of milk and other dairy products, and for use in the examination of water.

Violet Red Bile Agar is a selective medium used to detect and enumerate lactose-fermenting coliform microorganisms. American Public Health Association. Non-lactose-fermenting microorganisms produce colorless colonies.