Cette biodégradation est basée sur le fonctionnement d’un consortium bactérien complexe, composé de bactéries autotrophes et hétérotrophes, associées sous. Request PDF on ResearchGate | Influence possible des protozoaires sur le taux de mortalité des bactéries autotrophes nitrifiantes | Le modele de l’IAWQ du. Bactérie (Bacteria): Les bactéries sont des organismes vivants unicellulaires, très Les bactéries autotrophes telles que Nitrobacter, active dans les processus.

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Change the order of display of the official languages of Canada English first French first Option to display the non-official languages Spanish or Portuguese Neither Spanish Portuguese Display definitions, contexts, etc. Pure cultures of Nitrosomonas europaea and Ls winogradskyi were obtained from the National Collection of Industrial and Marine Bacteria Aberdeen, Scotland and a continuous enrichment culture of mixed heterotrophic bacteria, without nitrifying organisms, was obtained with a freshwater inoculum by imposing a residence time of 2 hours less than the generation time of nitrifying bacteria.

Classical culture techniques most probable number and immunofluorescence techniques are known to greatly underestimate the numbers of active organisms. They consist in measuring differences of ammonium, nitrite and nitrate dynamics in an inhibited and control sample during time. Switch to classic view. The language you choose must correspond to the language of the term you have entered.

The method can be used in in situ conditions, to allow real nitrifying activities measurements in samples. For this reason N-serve was added in the empty flask before the sample to allow the evaporation of the solvent. These experiments consisted in measuring the effect of aurotrophe combinations of N-serve, ethanol the organic solvent of N-serve and chlorate, on N-oxidation rates and C incorporation rates on samples of the two nitrifying enrichment cultures ammonium- and baxterie bacteria.


The inhibitors effects were also determined on the C incorporation rates of heterotrophic bacteria. This potential atuotrophe can be used to estimate the nitrifying biomass by considering a conversion factor of 0. Continuous enrichment cultures of nitrifying bacteria were obtained from an inoculum coming from the Seine estuary freshwater section.

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A good alternative to these methods consists in the measurement of potential nitrifying activity which is correlated to the nitrifying biomass. Nitrifying cell numbers and size auutotrophe the pure cultures were determined by epifluorescence with a microscope, after DAPI staining.

The organic solvent enhanced heterotrophic incorporation of C which totally masked out the autotrophic contribution of nitrifying bacteria. However, the ethanolic solution of N-serve had an unwanted result on C incorporation.

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To measure potential nitrifying activities, the optimal growth conditions of nitrifying bacteria were determined on enrichment cultures: Bicarbonate was measured by acid uatotrophe in natural water samples, and with the evolution method for culture samples. This factor is considered to be constant in the standard incubation conditions. Several methods were used for our study: These methods can be applied as long as the inhibitors are specific for nitrifying bacteria, and activities are high enough to allow the measurement of concentration variations during incubation times which are not too long.

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The measurement of the activity and biomass of bacferie bacteria is thus essential to sutotrophe and quantify the general nitrogen fluxes in those environments. This work presents a reassessment of the autotrophic nitrifying activity measurement by the 14C-bicarbonate incorporation method and its use to estimate the biomass of nitrifying bacteria. In this case, carbon incorporation rates can be converted in ammonium oxidation rates with the use of the conversion factor 0.

Biovolumes were estimated according to cell size and converted in biomasses according to a conversion factor determined experimentally with a carbon analyser.


Revue des sciences de l’eau11 2— The first methods developed were based on the use of specific nitrification inhibitors: By regenerating oxidised forms of bavterie nitratethe nitrification process plays an important role in the nitrogen cycle of aquatic environments. Writing tools A collection of writing tools that cover the many facets of English and French grammar, style and usage.

The method can also be used by placing baccterie sample in optimal temperature, pH, oxygen and ammonium conditions for nitrifying bacteria, to allow potential nitrifying activity measurements. Download the article in PDF to read it. The main differences of our protocol to the original ones is that we propose the use of a combination of 2 inhibitors, N-serve and chlorate, and the elimination by evaporation of the organic solvent of N-serve ethanol to avoid any interference with the heterotrophic populations.

The determined optimal growth rate was 0. Our conclusion is that the results presented in this paper allow the validation of the 14C-bicarbonate incorporation method with and without inhibitors to measure the nitrifying activity.

Ammonium was measured with the indophenol autogrophe method, nitrate was reduced in nitrite on a cadmium bed le nitrite was measured with the sulfanilamide method. Revue des sciences de l’eau 11, no.

By acting this way, inhibition of autotrophic C incorporation by nitrifying bacteria was also complete, while heterotrophic incorporation was unaffected. Recently developed gene-probes techniques work well for the identification of particular strains, but are not yet useful for led numeration.

Nitrifying activity, nitrifying biomass, 14C-bicarbonate incorporation, nitrification inhibitors, N-serve, chlorate.